Cycle no. | Amino acid | pmol |
|---|
1 | ND | – |
2 | D | 26.1 |
3 | L | 18.9 |
4 | P | 10.5 |
5 | Q | 15.3 |
- Purified rFeIFN-α15 was separated on a SDS-PAGE gel, and its N-terminal amino acid sequence was determined by five cycles of automated Edman degradation. The N-terminal sequences of the two main bands on SDS-PAGE were determined. Results obtained from analysis of the larger, 20-kDa band are shown; results obtained with the < 20-kDa band were identical. ND: No amino acid was detected during the first cycle of degradation, suggesting that it was cysteine
