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Fig. 5 | BMC Veterinary Research

Fig. 5

From: Novel recombinant feline interferon carrying N-glycans with reduced allergy risk produced by a transgenic silkworm system

Fig. 5

Glycosidase F digestion of rFeIFN-α15. rFeIFN-α15 was analysed by SDS-PAGE following glycosidase digestion in the presence (lanes 1, 4, and 7) or absence (lanes 2, 5, and 8) of SDS and NP-40 and in the absence of glycosidase (lanes 3, 6, and 9). One gel of each was stained with Coomassie Brilliant Blue (left panel; lanes 1, 2, and 3), whereas the others were treated with anti-FeIFN-α15 antibody (middle panel; lanes 4, 5, and 6) or Con A (right panel; lanes 7, 8, and 9)

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