Fig. 4From: Novel recombinant feline interferon carrying N-glycans with reduced allergy risk produced by a transgenic silkworm systemExtraction and purification of rFeIFN-α15 from cocoons. The crude extract obtained from the solubilisation of cocoon proteins with PBS (lane 1), the eluent from the Blue Sepharose™ column during the first step (lane 2), and the protein further purified on the ceramic hydroxyapatite column as the second step (lane 3) were analysed by SDS-PAGE followed by silver staining. The protein marker is shown as MBack to article page