Fig. 5
From: Co-localization of and interaction between duck enteritis virus glycoprotein H and L
Co-immunoprecipitation of pCMV-Flag-gL and pCMV-Myc-gH. a COS-7 cells were co-transfected with experimental group (pCMV-Flag-gL + pCMV-Myc-gH) and control groups (pCMV-Flag-gL + pCMV-Myc or pCMV-Flag-gL + pCMV-Myc-gH) respectively. The uses of antibodies were as following: IP: anti-gL or anti-Myc polyclonal antibody for immunoprecipitation; WB: anti-Myc or anti-Flag polyclonal antibody for Western-blotting. b Recombinant plasmids pCMV-Flag-gL and pCMV-Myc-gH were co-transfected into COS-7 cells. Line 1: cellular extracts that analyzed by Western-blotting through anti-gL mouse polyclonal antibody; Line 2: Extracts that analyzed through anti-Myc rabbit polyclonal antibody