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Table 1 Primers for PCR or sequencing

From: Safety and immunogenicity of an attenuated Chinese pseudorabies variant by dual deletion of TK&gE genes

Primer

Sequence(5′- 3′)

Positions

PRV ΔTK En pa F

CGGTATTTACGATGCGCAGACCCGGAAGCAGAACGGCAGCGCTCACGGCCCTGCGCAACGGGATGACGACGATAAGTAGGGATAAC

59,655

PRV ΔTK En pa R

GTTGACCAGCATGGCGTAGACGTTGCGCAGGGCCGTGAGCGCTGCCGTTCTGCTTCCGGGGGGTAATGCCAGTGTTACAACCA

60,081

PRV ΔTK check F

CGGATCTACCTCGACGGCGCCTA

59,524

PRV ΔTK check R

TTGTACGCGCCGAAGAGGGTGT

/

PRV BAC H1 F

GTACCCGTACACCGAGTCGT

121,170

PRV BAC H2 R

TTGTGGACCCGCGCGAACAT

126,648

PRV ∆gE check F

AGCCCCGGGAAGATAGCCAT

123,141

PRV ∆gE check R

ATCGCGGAACCAGACGTCGAAG

125,157

PRV gD part F

GGTGCGCGCACCTGCTGTACTTTA

121,385

PRV gD part R

AGATGTAGACGCACACGCCCACCAG

122,191

  1. Positions indicate the position of the first base of 5′ terminal on the reference genome sequence NC_006151.1. “/” indicate the primer is not on the genome of PRV but the Kanamycin resistance mark gene