Fig. 1From: Safety and immunogenicity of an attenuated Chinese pseudorabies variant by dual deletion of TK&gE genesConstruction of TK&gE dual deletion mutant virus (PRVΔTK&gE-AH02 strain). a A fragment with selection mark(sm) was amplified to target part of UL23(TK) gene in the genome of PRV. b Homologous recombination was conducted through En Passant protocol to delete part of TK gene. c The sm of kanamycin resistance gene was removed in the 2nd Red recombination. d Another homologous recombination was performed to recover the whole gI gene and part of gE gene during virus rescuing. e Schematic presentation of the TK&gE dual deletion mutant was shown. Scales in bp or kbp are providedBack to article page