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Fig. 1 | BMC Veterinary Research

Fig. 1

From: A novel approach to identifying and quantifying neutrophil extracellular trap formation in septic dogs using immunofluorescence microscopy

Fig. 1

Representative immunofluorescent images of cytology samples of bronchoalveolar lavage from a dog demonstrating the use of interference controls. Cells were fixed, permeabilized, and incubated with rabbit anti-human citrullinated histone H3 (citH3) antibody, followed by secondary goat anti-rabbit antibody conjugated with Alexa Fluor 568. After blocking with rabbit serum and unconjugated goat anti-rabbit Fab fragments, cells were incubated with goat anti-rabbit antibody conjugated with Alexa Fluor 488 without the second primary antibody against myeloperoxidase (MPO). Cells analysed under the TEXAS RED channel demonstrated presence of citH3 (red) (a), while no non-specific binding of secondary body conjugated to Alexa Fluor 488 was detected (b). DNA  was stained with DAPI (c) to ensure the colocalization of citH3 and DNA (d). Original 40× magnification

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BMC Veterinary Research

ISSN: 1746-6148

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