Primer | Primer sequence (5′-3′) | Product size |
---|---|---|
Cloning primers | ||
rrnBT1T2 | F:ACTAGTGTCAATGATGAGATCTGGCTGTTTTGGCGGATGAGAGA R:CTCGAGAGAGTTTGTAGAAACGCAAAAAGGC | 438 bp |
LFEC | F:TCTAGAGATCTCTCCTTCACAGATTCCCAAT R:CTCGAGAGAGTTTGTAGAAACGCAAAAAGGC | 1022 bp |
TF | F: GGCGCGCCTTAGGCGAGACCGTTCAT R:TTAATTAACGTATGACGCACTAGTCGATCAGGCTTGAAATGG | 1020 bp |
TR | F:ACTAGTGACTAGCGATCTCGAGCACGCATACAGGCACGTA R: TTAATTAAGGGTCACGAGCAAGGTAT | 1392 bp |
Screening primers | ||
thyA | F: TGTGGCATCACTTAGGACC R: TGTGGCATCACTTAGGACC | 3268 bp |
Real-time PCR primes | ||
PEDV-N | F:ACTGAGGGTGTTTTCTGGGTTGC R:GGTTCAACAATCTCAACTACACTGG | 137 bp |
Beta-actin | F:AAGGATTCATATGTGGGCGATG R:TCTCCATGTCGTCCCAGTTGGT | 103 bp |