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Table 2 Primers used in this study

From: Construction and characterization of thymidine auxotrophic (ΔthyA) recombinant Lactobacillus casei expressing bovine lactoferricin

Primer

Primer sequence (5′-3′)

Product size

Cloning primers

 rrnBT1T2

F:ACTAGTGTCAATGATGAGATCTGGCTGTTTTGGCGGATGAGAGA

R:CTCGAGAGAGTTTGTAGAAACGCAAAAAGGC

438 bp

 LFEC

F:TCTAGAGATCTCTCCTTCACAGATTCCCAAT

R:CTCGAGAGAGTTTGTAGAAACGCAAAAAGGC

1022 bp

 TF

F: GGCGCGCCTTAGGCGAGACCGTTCAT

R:TTAATTAACGTATGACGCACTAGTCGATCAGGCTTGAAATGG

1020 bp

 TR

F:ACTAGTGACTAGCGATCTCGAGCACGCATACAGGCACGTA

R: TTAATTAAGGGTCACGAGCAAGGTAT

1392 bp

Screening primers

thyA

F: TGTGGCATCACTTAGGACC

R: TGTGGCATCACTTAGGACC

3268 bp

Real-time PCR primes

 PEDV-N

F:ACTGAGGGTGTTTTCTGGGTTGC

R:GGTTCAACAATCTCAACTACACTGG

137 bp

 Beta-actin

F:AAGGATTCATATGTGGGCGATG

R:TCTCCATGTCGTCCCAGTTGGT

103 bp

  1. Enzyme restriction sites are underlined