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Fig. 5 | BMC Veterinary Research

Fig. 5

From: Selenium ameliorates Staphylococcus aureus-induced inflammation in bovine mammary epithelial cells by inhibiting activation of TLR2, NF-κB and MAPK signaling pathways

Fig. 5

Effect of Se on S. aureus-induced p38 and Erk phosphorylation in bMECs. Cells were pretreated with various concentrations (0, 2, 4 and 8 μM) of Se or serum-free medium for12 h before stimulated with S. aureus (MOI = 1:1) for 0.5 h and then washing twice with PBS. Total proteins were prepared at the indicated time points and subjected to Western blotting. Con = control cells without any treatment; mod = cells treated with S. aureus (MOI = 1:1) only; low = Se (2 μM) + S. aureus (MOI = 1:1); mid = Se (4 μM) + S. aureus (MOI = 1:1); high = Se (8 μM) + S. aureus (MOI = 1:1). The data are shown as mean ± sem (n = 3). ###: p < 0.001 vs. Con; *: p < 0.05 vs. Mod; **: p < 0.01 vs. Mod; ***: p < 0.001 vs. Mod. One out of 3 independent experiments is shown

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