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Fig. 3 | BMC Veterinary Research

Fig. 3

From: Selenium ameliorates Staphylococcus aureus-induced inflammation in bovine mammary epithelial cells by inhibiting activation of TLR2, NF-κB and MAPK signaling pathways

Fig. 3

The effect of Se on gene expression of pro-inflammatory cytokines induced by S. aureus in bMECs. Cells were incubated with various concentrations of Se or serum-free medium for 12 h and subsequently challenged with S. aureus (MOI = 1:1) for 0, 6, 8, and 10 h. Total RNA was prepared at the indicated time points after S. aureus injection. The TNF-α, IL-1β and IL-6 mRNA expression were quantified using qRT-PCR. con = control cells without any treatment; mod = cells treated with S. aureus (MOI = 1:1) only; low = Se (2 μM) + S. aureus (MOI = 1:1); mid = Se (4 μM) + S. aureus (MOI = 1:1); high = Se (8 μM) + S. aureus (MOI = 1:1). The data are shown as mean ± sem (n = 3). ##: p < 0.01 vs. Con; ###: p < 0.001 vs. Con; *: p < 0.05 vs. Mod; **: p < 0.01 vs. Mod; ***: p < 0.001 vs. Mod

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BMC Veterinary Research

ISSN: 1746-6148

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