Skip to main content
Fig. 2 | BMC Veterinary Research

Fig. 2

From: Selenium ameliorates Staphylococcus aureus-induced inflammation in bovine mammary epithelial cells by inhibiting activation of TLR2, NF-κB and MAPK signaling pathways

Fig. 2

The effect of Se on TLR2 signaling pathway associate genes induced by S. aureus in bMECs. Cells were incubated with different concentrations of Se or serum-free medium for 12 h and subsequently treated with S. aureus (MOI = 1:1) for 0, 6, 8 and 10 h. Total RNA was prepared at the indicated time points after S. aureus treated. The TLR2 (a) , Myd88 (b) , Irak4 (c) , Irak1 (d) and Traf6 (e) mRNA expression levels were assayed using qRT-PCR. con = control cells without any treatment; mod = cells treated with S. aureus (MOI = 1:1) only; low = Se (2 μM) + S. aureus (MOI = 1:1); mid = Se (4 μM) + S. aureus (MOI = 1:1); high = Se (8 μM) + S. aureus (MOI = 1:1). The data are shown as mean ± sem (n = 3). ###: p < 0.001 vs. Con; *: p < 0.05 vs. Mod; **: p < 0.01 vs. Mod; ***: p < 0.001 vs. Mod

Back to article page

BMC Veterinary Research

ISSN: 1746-6148

Contact us