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Table 5 Detection PEDV in pooled faecal samples by RtF-RT-LAMP

From: Development of pooled testing system for porcine epidemic diarrhoea using real-time fluorescent reverse-transcription loop-mediated isothermal amplification assay

Pooled size

5

10

15

20

25

30

35

40

45

50

331

+

+

+

–

–

–

–

–

–

–

M1

+

+

+

+

+

+

+

+

+

–

329

+

+

+

+

+

+

+

+

+

+

324

+

+

+

+

+

+

+

+

+

+

  1. Pooled size: Each PEDV positive sample was pooled with PEDV negative samples in different pooling ratios including 1:4, 1:9, 1:14, 1:19, 1:24, 1:29, 1:34, 1:39, 1:44, and 1:49
  2. 331, M1, 329, 324: Four positive samples were selected for pooling with a different number of negative samples
  3. +: Positive by RtF-RT-LAMP
  4. -: Negative by RtF-RT-LAMP