Pooled size | 5 | 10 | 15 | 20 | 25 | 30 | 35 | 40 | 45 | 50 |
---|
331 | + | + | + | – | – | – | – | – | – | – |
M1 | + | + | + | + | + | + | + | + | + | – |
329 | + | + | + | + | + | + | + | + | + | + |
324 | + | + | + | + | + | + | + | + | + | + |
- Pooled size: Each PEDV positive sample was pooled with PEDV negative samples in different pooling ratios including 1:4, 1:9, 1:14, 1:19, 1:24, 1:29, 1:34, 1:39, 1:44, and 1:49
- 331, M1, 329, 324: Four positive samples were selected for pooling with a different number of negative samples
- +: Positive by RtF-RT-LAMP
- -: Negative by RtF-RT-LAMP