Development of pooled testing system for porcine epidemic diarrhoea using real-time fluorescent reverse-transcription loop-mediated isothermal amplification assay

Table 1 Primers used for RtF-RT-LAMP in this study

Primer	ID	Sequence (5′ to 3′)	Gene location
F3	PED_F3_ID1	TCCTTATGGCTTGCATCAC	25,846–25,864
B3	PED_B3_ID1	CCGTAGACAATTGTTGTAGTGG	26,143–26,122
FIP	PED_FIP_ID1, PED_FIP_ID1modified	GTMGGCCCATCACAGAAGTAGTTTT GGTTGTGGCGCAGGACA	25,983–25,963 (TTTT) 25,903–25,919
BIP	PED_BIP_ID1	CCAACTGGTGTAACGCTAACACTTTTT TACCTGTACGCCAGTAGC	26,010–26,032 (TTTT) 26,087–26,070
LF	PED_LF_ID37	TTTCAGGATTGAAAGACCACCAAG	25,947–25,924
LB	PED_LB_ID6	GGTACATTGCTTGTAGAGGGCTATAA	26,040–26,065

ISSN: 1746-6148