Table 2 The primer list for reverse transcription and amplification of RT-qPCR
Gene | NCBI Accession | PCR product | Forward Primer (5’-3’) | Reverse Primer (5′ -3’) |
|---|---|---|---|---|
H1R | NM_174083.4 | 245 bp | AGCCAGAACCAGCTTGAGAT | TTCATGTGCAAGCCAGACAC |
H2R | XM_015464941.1 | 198 bp | TTGGCAAGGTCTTCTGCAAC | GGAAGGACAGGGTGATGGAA |
H3R | XM_015466045.1 | 172 bp | CAGAAGATGGTGCTGGTGTG | AAGGGGTGAAGAACTCGAGG |
H4R | XM_001251983.5 | 221 bp | ATCCTTGCCCTCACGTTAGT | CGAAGGGATGCTGCTGATTC |
IL-1β | NM_174093.1 | 167 bp | GGCCAAAGTCCCTGACCTCT | CTGCCACCATCACCACATTC |
TNF-α | NM_173966.3 | 261 bp | CACATACCCTGCCACAAGGC | CTGGGGACTGCTCTTCCCTCT |
NF-κB | NM_001045868.1 | 129 bp | ATACGTCGGCCGTGTCTAT | GGAACTGTGATCCGTGTAG |
mTOR | XM_001788228.1 | 199 bp | ATGCTGTCCCTGGTCCTTATG | GGGTCAGAGAGTGGCCTTCAA |
P70S6K | NM_205816.1 | 162 bp | GGACATGGCAGGGGTGTTT | GGTATTTGCTCCTGTTACTTTTCG |
4EBP1 | BC120290.1 | 177 bp | GGCAGGCAGTGAAGAGTC | CCTGGGCTGCGGGAT |
αS1-casein | NM_181029.2 | 170 bp | ATTTTCAGACAATTCTACCAGCT | AATTCACTTGACTCCTCACCAC |
GAPDH | NM_001034034.2 | 135 bp | TGTTGTGGATCTGACCTGCC | AAGTCGCAGGAGACAACCTG |