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Table 1 Primer sequences for amplification of ORF5 and ORF7

From: Geographic distribution and molecular analysis of porcine reproductive and respiratory syndrome viruses circulating in swine farms in the Republic of Korea between 2013 and 2016

Type

Primer

Product (bp)

 

Position

Sequence

1

Forward

14,653–14,671 (ORF7)

5’-ATGGCCAGCCAGTCAATCA-3’

ORF7 (398)

Reverse

15,030-15,050 (3’NCR)

5’-TCGCCCTAATTGAATAGGTGA-3’

Forward

13,444–13,461 (ORF4)

5’-AATGAGGTGGGCYACAACC-3’

ORF5 (754)

Reverse

15,030–15,050 (ORF6)

5’-GCGTGACACCTTAAGGGC-3’

2

Forward

14,933–14,951 (ORF7)

5’-ATGGCCAGCCAGTCAATCA-3’

ORF7 (433)

Reverse

15,346–15,365 (3’NCR)

5’-TCGCCCTAATTGAATAGGTGA-3’

Forward

13,759–13,778 (ORF4)

5’-CCATTCTGGTGGCAATTTGA-3’

ORF5 (716)

Reverse

14,455–14,474 (ORF6)

5’-GGCATATATCATCACTGGCG-3’