Fig. 5
Viral loads in tissues collected from guinea pigs infected with the four virus strains at 3 dpi for the inoculated group and 5 dpe for the contact group. Guinea pigs were inoculated with 106 EID50/mL of the JS/10, KR/07, MV/12 and CA/09 strains. Organs including the lung, trachea, brain, nasal turbinate, soft palate and rectal were collected for the determination of the viral loads using real-time PCR at 3 dpi and 5 dpe, for the inoculated group (a) and contact group (b) for each virus, respectively. The results are expressed as log10 (viral RNA copies)/g. *, P < 0.05, or **, P < 0.01, indicates significantly different virus titers compared between JS/10 and KR/07 virus group. #, P < 0.05, or ##, P < 0.01, indicates a significant difference in virus load for the group MV/12 compared with group KR/07. For viral loads in different organs mentioned above, the results are expressed in terms of mean virus titer logEID50. The horizontal line means the detection limit of this assay (158 copies of RNA per g)