Table 2 Primers used for real time PCR
Primer | Sequence 5′ - > 3′ | Fragment size (bp) | Accession number |
|---|---|---|---|
CD3 F | GCAATTTACGATGATCCCAGAG | 112 | XM_005500716.2 |
CD3 R | GCGTCCACTTCAATGCAATTC | ||
CD4 F | GAACGTGTGAATGGGACTCAGA | 116 | MG214789 |
CD4 R | GTCATTGTCTTCTATGAGGTGACA | ||
CD8 F | TTCATCTGGGTTCCCTTGGCA | 97 | MG214790 |
CD8 R | CTGCATCTTCGGCTCCTGGT | ||
IFNγ F | CTGACAAGTCAAAGCCGCAC | 125 | DQ479967.1 |
IFNγ R | AGTCATTCATCT GAAGCTTGGC | ||
GAPDH F | CCCTGAGCTCAATGGGAAGC | 137 | NM_001282835.1 |
GAPDH R | TCAGCAGCAGCCTTCACTAC |