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Fig. 2 | BMC Veterinary Research

Fig. 2

From: In vitro inhibition of porcine reproductive and respiratory syndrome virus replication by short antisense oligonucleotides with locked nucleic acid modification

Fig. 2

Cell viability analysis of Marc-145 cells transfected with AS-ONs containing LNA modifications. The cell viability was assayed 72 h post transfection. The measurement of each concentration was normalized to the corresponding cell control (as 100%). Compared with the cell control, both DNA AS-ON (YN8, when concentration ≥ 48 μM) and the AS-ONs with LNA modification (LNA-YN8-A and LNA-YN8-B, when concentration ≥ 56 μM) showed some cytotoxicity. Higher concentration led to higher cytotoxicity. However, LNA modification conferred lower cytotoxicity when compared with the corresponding DNA AS-ON YN8. No difference in cell viability was observed between LNA-YN8-A and LNA-YN8-B. Shown here are the representative data from three independent experiments. NS: not significant; *: P < 0.05; **: P < 0.01

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BMC Veterinary Research

ISSN: 1746-6148

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