Skip to main content
Fig. 1 | BMC Veterinary Research

Fig. 1

From: In vitro inhibition of porcine reproductive and respiratory syndrome virus replication by short antisense oligonucleotides with locked nucleic acid modification

Fig. 1

AS-ONs with LNA modifications were present in cell culture twenty hours post transfection. Cy3-labelled antisense oligonucleotides (YN8, LNA-YN8-A and LNA-YN8-B, 32 μM) were used in the transfection in Marc-145 cells. The fluorescent signals were recorded with fluorescence microscopy (Olympus) at the indicated time points post transfection. The fluorescent signals decreased over the time, however, compared with the DNA AS-ON YN8, we could clearly observe the signals even 20 h post transfection, indicating that incorporating LNA into the antisense oligonucleotide could improve the stability in Marc-145 cells for sufficiently longer time periods to induce the degradation of viral RNA and thus to inhibit PRRSV replication. Shown here are the representative images from three independent experiments

Back to article page