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Table 3 Isolation of H9N2 viruses in vaccinated chickens after challenged with the passaged viruses

From: Evolution of H9N2 avian influenza virus in embryonated chicken eggs with or without homologous vaccine antibodies

Group

Challenge virus

HI mean titer(2n)

3dpi

5dpi

(Homologous antigen 2n)

Tracheal swaba (Virus titers b)

Cloacal swaba (Virus titersb)

Tracheal swaba (Virus titers b)

Cloacal swaba (Virus titers b)

A (None-immune)

BSG

0

0/6

0/6

0/6

0/6

F/98

0

6/6 (3.2 ± 0.3)

1/6 (1.6 ± 0.1)

3/6 (1.8 ± 0.2)

0/6

nF47

0

6/6 (5.2 ± 0.1)

6/6 (3.1 ± 0.3)

2/6 (3.2 ± 0.1)

2/6 (1.1 ± 0.1)

vF47

0

6/6(4.1 ± 0.2)

3/6 (2.1 ± 0.1)

6/6 (1.5 ± 0.3)

2/6 (<1C)

nF52

0

6/6 (3.6 ± 0.1)

2/6 (3.8 ± 0.2)

6/6 (4.1 ± 0.2)

2/6 (1.6 ± 0.3)

B (Immune F/98)

F/98

7

1/6 (1.2 ± 0.1)

0/6

0/6

0/6

nF47

7

1/6(3.1 ± 0.1)

0/6

2/6 (1.3 ± 0.3)

0/6

vF47

7.3

6/6 (4.3 ± 0.1)

4/6 (2.2 ± 0.2)

6/6 (2.7 ± 0.2)

5/6 (1.0 ± 0.2)

nF52

7

6/6 (3.9 ± 0.3)

2/6 (1.6 ± 0.1)

6/6 (2.1 ± 0.1)

3/6 (<1C)

C (immune nF47)

F

7

0/6

0/6

0/6

0/6

 

nF47

7

0/6

0/6

0/6

0/6

D (immune vF47)

F

6.7

0/6

0/6

0/6

0/6

vF47

6.7

0/6

0/6

0/6

0/6

E (immune nF52)

F

7

1/6(1.9 ± 0.3)

0/6

0/6

0/6

nF52

7

0/6

0/6

0/6

0/6

  1. anumber of positive tracheal or cloacal swabs /total number of chickens
  2. bVirus titers in tracheal or cloacal swabs are expressed as mean log10 EID50/ml ± SD
  3. cLower than the detection limit of 10 EID50