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Table 1 Primary antibodies used for immunofluorescence and western blotting

From: Characterisation of feline renal cortical fibroblast cultures and their transcriptional response to transforming growth factor β1

Antibody

Supplier

Secondary antibody

Dilution for IF

Dilution for western blot

Incubation temperature for western blot

Cytokeratin AE1/AE3

Dako

Mouse

1:100

1:1000

4 °C

Vimentin

(V9)

Dako

Mouse

1:100

1:1000

4 °C

Desmin

(DE-R-11)

Dako

Mouse

1:100

N/A

N/A

CD29

(TS2/16)

Bio-rad

Mouse

1:100

1:500

4 °C

CD44

(156-3C11)

ABD serotec

Rat

1:100

N/A

N/A

vWF

Dako

Rabbit

1:200

1:1000

4 °C

VCAM-1

R&D

Mouse

1:100

N/A

N/A

Rabbit Polyclonal Isotype control

Biolegend

Rabbit

1:50–1:200

N/A

N/A

Mouse IgG1 κ isotype control

Biolegend

Mouse

1:50–1:200

N/A

N/A

β-actin

(AC-74)

Sigma-Aldrich

Mouse

N/A

1:5000

4 °C