Fig. 6

Reversing the effects of MβCD on PRV entry using exogenous cholesterol. MA104 cells (PRV) and BHK-21 cells (VSV) were pretreated with 15 mM MβCD at 37 °C for 30 min, then replenished 200 μM exogenous cholesterol at 37 °C for 1 h. Treated cells were then infected with PRV or VSV (100 TCID₅₀), respectively, and the homogenates examined by western blot analysis and virus titers. a Virus titer of PRV and VSV after treatment. b Western blot of PRV VP7 and VP4 proteins and β-actin. c Western blot of VSV G protein and β-actin. Western blots depict representative experiments. Error bars (Fig. 6a) indicate the standard deviations of three independent experiments