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Fig. 1 | BMC Veterinary Research

Fig. 1

From: Cholesterol of lipid rafts is a key determinant for entry and post-entry control of porcine rotavirus infection

Fig. 1

PRV infection inhibited by cholesterol depletion with MβCD. MA104 cell and BHK-21 cell monolayers were treated with increasing concentrations of MβCD before virus infection. Cells were subsequently, infected with PRV or VSV (100 TCID50) respectively, except “mock” as control without virus infection and MβCD treatment. a IFA data demonstrating infectivity of PRV and VSV using anti-VP7 of PRV antibodies and anti-G protein of VSV antibodies respectively. The 100% infectivity values of PRV and VSV represent average fluorescein-stained cells numbers of 100 and 240, respectively. b Real-time PCR data for PRV and VSV targeting the VP7 gene and G-protein. c Western blot analysis using antibodies to VP7 for PRV, G-protein for VSV and β-actin served as a protein loading control. Error bars indicate the standard deviations of three independent experiments

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