Fig. 5

Inhibitory effects of cortisol on LPS-stimulated NF-κB p65 and IκBα phosphorylation in RAW 264.7 cells. a Cells were stimulated with LPS (1 μg/mL) alone for 0, 15, 30 and 45 min. b Cells were co-treated with cortisol (5, 15 and 30 ng/mL) and LPS (1 μg/mL) for 30 min. Total proteins were isolated and subjected to Western blotting. The data presented are the means±SEM. ** p < 0.01 vs. the control group; # p < 0.05, ## p < 0.01 vs. the LPS group