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Table 2 Primers used

From: Molecular analysis of carnivore Protoparvovirus detected in white blood cells of naturally infected cats

 

Primer name

Primer sequence

Nucleotide position

Fragment amplified

Real Time-PCR

A-for

5’-AGCTACTATTATGAGACCAGCTGAG-3′

3767–3791

A-B: 99 bp

B-rev

5′- CCTGCTGCAATAGGTGTTTTAA-3’

3844–3865

Hemi-nested-PCR

C-for (P3b)

5’-CCATTTCTAAATTCTTTG −3’

3651–3668

C-D: 881 bp

D-rev (VPreva)

5’-TTTCTAGGTGCTAGTTGAG −3’

4513–4531

E-rev (P4b)

5’-AAGTCAGTATCAAATTCTT −3’

4201–4219

C-E: 569 bp

  1. The primer positions refer to the nucleotide sequence of FPV reference strain CU-4 (GenBank accession number M38246). [41]a, [42]b