Table 3 Mean quantification cycle (Cq) values resulting from MGB PCRa
From: A rapid minor groove binder PCR method for distinguishing the vaccine strain Brucella abortus 104M
Detection of 104M genomic DNA | Detection of A19 genomic DNA | ||||
|---|---|---|---|---|---|
Concentration | Cq of FAM | Cq of VIC | Concentration | Cq of FAM | Cq of VIC |
1.1 ng/μL | 24.06 | NA | 3.8 ng/μL | NA | 17.49 |
110 pg/μL | 27.20 | NA | 380 pg/μL | NA | 20.89 |
11 pg/μL | 30.92 | NA | 38 pg/μL | NA | 24.39 |
1.1 pg/μL | 35.27 | NA | 3.8 pg/μL | NA | 31.90 |
110 fg/μL | 38.25 | NA | 380 fg/μL | NA | 34.69 |
11 fg/μL | NA | NA | 38 fg/μL | NA | 38.11 |
1.1 fg/μL | NA | NA | 3.8 fg/μL | NA | NA |
0.11 fg/μL | NA | NA | 0.38 fg/μL | NA | NA |