Development and application of an indirect enzyme-linked immunosorbent assay based on recombinant capsid protein for the detection of mink circovirus infection

Table 1 Ratio of MiCV-positive serum to MiCV-negative serum for the optimization of assay conditions for the ELISA against recombinant rCap antigen

Serum dilution	Concentration of rCap antigen (μg/well)
Serum dilution	4	2	1	0.5	0.25	0.125
1:25(+)	0.806	1.056	1.048	0.969	0.89	0.742
1:25(−)	0.108	0.166	0.16	0.153	0.133	0.104
1:50(+)	0.876	1.175	1.044	0.967	0.888	0.78
1:50(−)	0.175	0.116	0.144	0.12	0.116	0.09
1:100(+)	0.753	1.078	1.063	0.964	0.895	0.737
1:100(−)	0.096	0.121	0.129	0.1	0.114	0.089
1:200(+)	0.971	1.119	1.065	1.004	0.801	0.767
1:200(−)	0.113	0.127	0.15	0.108	0.097	0.088
1:400(+)	0.681	0.724	0.807	0.779	0.821	0.638
1:400(−)	0.094	0.068	0.111	0.083	0.083	0.068

ISSN: 1746-6148