Skip to main content

Table 2 Primer sets used for the detection of virulence genes in Streptococcus spp.

From: Biofilm production and other virulence factors in Streptococcus spp. isolated from clinical cases of bovine mastitis in Poland

Primer function

Putative function

Target gene

Primer sequence (5′–3′)

Amplicon size (bp)

Annealing temperature (°C)

Reference

Virulence genes

S.uberis

Adhesion molecue-

Adherence and invasion of mammary epithelial cells

sua

TCAACTTGACGAATCGCTTG

TCAGCCATTGTTTCTGCTTG

480

47

[20]

Plasminogen activator

colonization

pauA/skc

CGGGTTGAAGAACCTATCACTC

TGGAAGTTGACCAAGAGAATTG

255

50

[20]

Surfacedehydrogenase protein

colonization

gapC

GCTCCTGGTGGAGATGATGT

GTCACCAGTGTAAGCGTGGA

200

51

[11]

CAMP factor

Forms pores in host-cell membran

cfu

TATCCCGATTTGCAGCCTAC

CCTGGTCAACTTGTGCAACTG

205

50

[11]

Lactoferrin binding protein

Binding Lactoferrin

lbp

CGACCCTTCAGATTGGATTC

TAGCAGCATCACGTTCTTCG

698

50

[11]

Hyaluronic acid capsule

Resistance to phagocytosis

hasA

GAAAGGTCTGATGCTGATG

TCATCCCCTATGCTTACAG

319

44

[33]

hasB

TCTAGACGCCGATCAAGC

TGAATTCCTATGCGTCGATC

532

47

[33]

hasC

TGCTTGGTGACGATTTGATG

GTCCAATGATAGCAAGGTCAC

225

47

[33]

S.agalactiae

Adherence and virulence protein A

Binding to immobilized fibronectin

pavA

TTCCCATGATTTCAACAACAAG

AACCTTTTGACCATGAATTGGTA

495

47

[20]

Streptococcal C5a peptidase- adhesion

Prevents neutropil, promotes adherence

scpB

AGTTGCTTCTTACAGCCCAGA

GGCGCAGACATACTAGTTCCA

567

51

[20]

Surface protein Rib-

Resistance to proteases

rib

CAGGAAGTGCTGTTACGTTAAAC

CGTCCCATTTAGGGTCTTCC

369

51

[34]

C beta protein

Binds to the immuno-globulin A

bac

CTATTTTTGATATTGACAATGCAA

GTCGTTACTTCCTTGAGATGTAAC

592

46

[35]

Β-haemolisin

Promotes invasion of host cells

cylE

TGACATTTACAAGTGACGAAG

TTGCCAGGAGGAGAATAGGA

248

47

[36]

C alfa protein

Adherence to epithelial cells

bca

TAACAGTTATGATACTTCACAGAC

ACGACTTTCTTCCGTCCACTTAGG

535

49

[37]

CAMP factor

Forms pores in host-cell membran

cfb

ATGGGATTTGGGATAACTAAGCTAG

AGCGTGTATTCCAGATTTCCTTAT

193

50

[38]

Lamining-binding protein

Promote adherence to host laminin

lmb

AGTCAGCAAACCCCAAACAG

GCTTCCTCACCAGCTAAAACG

397

50

[39]

S.dysgalactiae

α-enolase

Plasminogen binding

eno

ATGTCAATTATTACTGATGT

CTATTTTTTTAAGTTATAGA

1308

36

[17]

Nephritis-associated plasminogen-binding receptor

Plasminogen binding

napr

GTTAAAGTTGGTATTAACGGT

TTGAGCAGTGTAAGACATTTC

963

45

[17]

Back to article page

BMC Veterinary Research

ISSN: 1746-6148

Contact us