Table 2 Enrichment of phage peptides from library by BEFV G1 protein biopanning
Item | First round biopanning | Second round biopanning | Third round biopanning | Fourth round biopanning |
|---|---|---|---|---|
G1(μg) | 10 | 7.5 | 5 | 2.5 |
Concentration of Tween20 (v/v) | 0.1% | 0.25% | 0.5% | 0.5% |
Input/PFU (pfu/ml) | 1 × 1011 | 1 × 1011 | 1 × 1011 | 1 × 1011 |
Output/PFU (pfu/ml) | 3.7 × 106 | 5.2 × 107 | 8.7 × 107 | 7.2 × 108 |
Recovery (Output/input) | 3.7 × 10−5 | 5.2 × 10−4 | 8.7 × 10−4 | 7.2 × 10−3 |