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Table 2 Sequences of oligonucleotide primers used for qPCR analysis of jejunum tissue

From: Birth delivery method affects expression of immune genes in lung and jejunum tissue of neonatal beef calves

Gene IDa

Primers (5′-3′)

Accession No.

bp2

NR3C1

Forward: TACAGGCAGCAATGGTCTCA

Reverse: AAGAGGGTGGTCATTCTGGG

NM_001206634.1

154

MUC1

Forward: CCCAACTCTGTTCTGGGCTA

Reverse: TTCCAGCCAGTATTCCAGCA

AJ400824.1

163

MUC2

Forward: TGCTACTACGTGCTGACCAA

Reverse: ACGTTCTTCTTGTTGTCGGC

XM_010806231.2

131

TNF- α

Forward: CGTGGACTTCAACTCTCCCT

Reverse: GGACACCTTGACCTCCTGAA

NM_173966.3

179

Il-1 β

Forward: CCAGCTGCAGATTTCTCACC

Reverse: TCACACAGAAACTCGTCGGA

NM_174093.1

195

il-4

Forward: TGCCCCAAAGAACACAACTG

Reverse: GAACAGGTCTTGCTTGCCAA

NM_173921.2

144

Il-6

Forward: ACTTCTGCTTTCCCTACCCC

Reverse: TGTCGACCATGCGCTTAATG

NM_173923.2

121

VIP

Forward: TCGACTCCCAGGACTTCAAC

Reverse: GAGAAGAGCACGCTGAACAG

NM_173970.3

148

FCGRT

Forward: ACTATCGCTCGCTCCAGTAC

Reverse: CCTGCGCCCGTAGATTATTG

NM_176657.1

126

B2M

Forward: GTTCACTCCCAACAGCAAGG

Reverse: TCTCGATGGTGCTGCTTACA

NM_173893.3

109

RAB11A

Forward: GCAACAAGAAGCATCCAGGT

Reverse: TAAGGCACCTACAGCTCCAC

NM_001038162.2

120

RAB25

Forward: AGCTGAGAGTTGAGGGCATT

Reverse: TCGGCTCTGTTTCCCATCTT

NM_001017936.1

102

STX3

Forward: GAGCAGCATCAAGGAGCTTC

Reverse: TACCAATTTCTTCCGGGCCT

NM_001101971.1

181

pIg

Forward: GGTGTGCTGGTTCCTTCTTG

Reverse: ATAAGCTGCAGGTGGGAACT

NM_174143.1

93

MYO5B

Forward: GAAGCAATACCGCATGCAGA

Reverse: TTCTGGATGATGGTGGCCTT

XM_015469176.1

147

  1. a NR3C1 Nuclear receptor subfamily 3 group C member 1, MUC1 Mucin 1, MUC2 Mucin 2, TNFα Tumor necrosis factor alpha, IL-1β Interleukin 1 beta, IL-4 Interleukin 4, IL-6 Interleukin 6, VIP Vasoactive intestinal polypeptide, FCGRT fc fragment of Ig receptor and transporter, B2M Beta −2- microglobulin, RAB11A Ras related protein Rab11a, RAB25 Ras related protein RAB25, STX3 Syntaxin 3, pIg Polymeric immunoglobulin receptor, MYO5B Myosin VB