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Table 2 Protein identification of 2D–spots shown in Fig. 4 by MALDI-TOF/TOF mass spectrometry

From: Influence of different sample preparation strategies on the proteomic identification of stress biomarkers in porcine saliva

Spot ID

Acc No.

Protein identified*

MW

pI

Mascot Score

N

Cov%

m/z

Range

Peptide Score

Peptide Sequence

20

P81608

Salivary lipocalin

21.6

5

107.1

2

8.9

899.5607

1291.6910

59–65

132–141

35.2

71.9

R.VFVEHIR.V

K.TFQLMEFYGR.K

76

gi|545882006

Prolactin-inducible protein

16.8

5.1

311.8

4

27.3

1318.7711

1449.6481

1947.1172

1964.1477

111–122

89–99

123–140

123–140

47.8

49.6

97.3

117

K.SMVITATVDIIR.Q

K.YTSCLCDDYPR.K

R.QLGICPQDQAVIPIAANR.F

R.QLGICPQDQAVIPIAANR.F

12

gi|545882006

Prolactin-inducible protein

16.8

5.1

355.9

4

27.3

1318.7716

1449.6317

1947.0736

1964.1255

111–122

89–99

123–140

123–140

66.8

74.5

91.8

122.9

K.SMVITATVDIIR.Q

K.YTSCLCDDYPR.K

R.QLGICPQDQAVIPIAANR.F

R.QLGICPQDQAVIPIAANR.F

26

gi|545882006

Prolactin-inducible protein

16.8

5.1

395.8

4

27.3

1318.7446

1449.6003

1947.0135

1964.1257

111–122

89–99

123–140

123–140

63

75.2

114.1

143.6

K.SMVITATVDIIR.Q

K.YTSCLCDDYPR.K

R.QLGICPQDQAVIPIAANR.F

R.QLGICPQDQAVIPIAANR.F

5

P01965

Hemoglobin subunit alpha

15

9.6

145.9

2

22

1422.6998

1876.8757

17–31

41–56

64.3

81.5

K.VFGGQAGAHGAEALER.M

K.TYFPHFNLSHGSDQVK.A

  1. Acc. No. = accession number. MW = molecular weight in kDa. pI = isoelectric point. N = number of peptides. Cov = sequence coverage. * Statistically significant identification P < 0.05. Theoretical pI and Mw values were sourced from the ExPASy Proteomics Server (Swiss Institute of Bioinformatics, Geneva)