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Table 1 Primer sets used to amplify DNA fragments specific for Babesia bovis

From: In silico and phylogenetic analyses of partial BbRAP-1, BbCP2, BbSBP-4 and BbβTUB gene sequences of Babesia bovis isolates from cattle in South Africa

Gene

Assay

Primer name

Oligonucleotide primers (5′ → 3′)

Annealing

Product sizea

Reference

BbRAP-1

PCR

BoF

F-CACGAGGAAGGAACTACCGATGTTGA

55 °C

360 bp

[12]

BoR

R-CCAAGGAGCTTCAACGTACGAGGTCA

[12]

nPCR

BoFN

F-TCAACAAGGTACTCTATATGGCTACC

57 °C

298 bp

[12]

BoRN

R-CTACCGAGCAGAACCTTCTTCACCAT

[12]

BbCP2

PCR

CpBovF

F-TGCATCGGACCTATCCAACC

57 °C

960 bp

This study

CpBovR

R-TCAGCAGCCAAATAAGGCCA

This study

nPCR

CpBov3

F-ATCGGAAGAAGTCGCCGTTG

65 °C

829 bp

This study

CpBov4

R-AAGCGTAGTCGCTGTAACCA

This study

BbSBP-4

PCR

BbSBP1

F-AGTTGTTGGAGGAGGCTAAT

57 °C

887–905 bp

This study

BbSBP2

R-CTTCTCGGCGTCCTTTTC

This study

nPCR

BbSBP3

F-CCGCATTCTTAAGACTTCTGA

60 °C

726–744 bp

This study

BbSBP4

R-GTTACCATTTCATCGTTGTCA

This study

BbβTUB

PCR

BTbovA

F-AGAGCGGTACTTACCACGGA

61 °C

1203 bp

This study

BTbovB

R-CGTCGTCGATGGTTGCTTCT

This study

nPCR

BTbovC

F-GTTCCACGCGCTGTACTCAT

65 °C

954 bp

This study

BTbovD

R-CATGTCCTGGATGGCGGTAG

This study

  1. aTheoretical product sizes based on nucleotide gene sequences of several B. bovis strains used as templates for primer design