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Fig. 1 | BMC Veterinary Research

Fig. 1

From: Performance of Leishmania PFR1 recombinant antigen in serological diagnosis of asymptomatic canine leishmaniosis by ELISA

Fig. 1

Expression and purification of L. infantum recombinant PFR1 protein. a Analysis by SDS-PAGE and Coomassie blue staining of the protein purification process. The Escherichia coli M15 strain was chosen as the host bacterium for LiPFR1 overexpression (lane 1). An intense band of approximately 70 kDa was observed after IPTG induction (lane 2) and not seen in uninduced cultures (lane 1). Purified LiPFR1 protein after purification by Ni2+ affinity chromatography is shown in lane 3. MW, molecular weight marker (kDa). b Western blot analysis of L. infantum PFR1 recombinant protein by using α-PFR2 antibody against the homologous PFR2 protein from T. cruzi. MW, molecular weight marker (kDa)

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BMC Veterinary Research

ISSN: 1746-6148

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