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Fig. 4 | BMC Veterinary Research

Fig. 4

From: Curcumin is a promising inhibitor of genotype 2 porcine reproductive and respiratory syndrome virus infection

Fig. 4

Measurement of a curcumin effect on PRRSV absorption in Marc-145 cells. Marc-145 cells were pre-chilled at 4 °C for 1 h followed by incubation with curcumin-pretreated GD-HD (MOI: 100) (a), or a mixture of GD-HD (MOI: 100) and curcumin (c) at 4 °C for 1 h. For pretreatment of cells, Marc-145 cells were pre-incubated with curcumin at 37 °C for 1 h. After incubating cells at 4 °C for 1 h, both GD-HD (MOI: 100) and curcumin were added and incubated for 1 h at 4 °C (b). After washing out unbound virus, total RNA was extracted from the cells and the relative cell-bound PRRSV RNA levels were measured using qRT-PCR

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