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Fig. 4 | BMC Veterinary Research

Fig. 4

From: Non-linear optical microscopy of cartilage canals in the distal femur of young pigs may reveal the cause of articular osteochondrosis

Fig. 4

Indication of calcification around a cartilage canal. Cyan: Two-photon-excited fluorescence (TPEF). Glowing red: Second harmonic generation (SHG). a Cross section of a cartilage canal with variable presence of intense TPEF signal in the adjacent cartilage matrix. The intense TPEF signal is only located around the canal segment surrounded by hypertrophic chondrocytes, and the segment running into the image plane (asterisk) has no intense TPEF adjacent to it. b Close-up image of the dashed rectangle in a. c The intense TPEF of the calcified zone near the ossification front is shown for comparison. The TPEF signal is therefore presumably an indication of calcification. 25×–focusing objective was used to generate all images. The bars equal 250 μm in a and 100 μm in b and c

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