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Fig. 4 | BMC Veterinary Research

Fig. 4

From: Extensive protein hydrolyzation is indispensable to prevent IgE-mediated poultry allergen recognition in dogs and cats

Fig. 4

SDS-PAGE (a) and immunoblotting (b). a: Extracts (5 μg/lane) were separated in 4–12% gels by SDS-PAGE. b: Western immunoblotting of the same extracts. The blot was incubated with a chicken-reactive dog serum pool at a 1:10 dilution and revealed with anti-dog IgE-AP at 0.5 μg/mL followed by NBT/BCIP revelation. After incubation with this dog serum pool, a 48 kDa protein band was found to be the target of IgE in the CMT and BMT extracts; bands were not visible with the other extracts, however

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BMC Veterinary Research

ISSN: 1746-6148

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