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Table 1 Results of the analysis of boot swabs and liquid manure samples (n/ %) from 77 herds by faecal culture in three labs (I to III) and by two different qPCR methods (qPCR A and qPCR B) for Mycobacterium avium ssp. paratuberculosis (MAP)

From: Evaluation of different diagnostic methods for the detection of Mycobacterium avium subsp. paratuberculosis in boot swabs and liquid manure samples

Methode of MAP detection

Laba

Boot swab

Liquid manure

posb

negc

n.a.d

pos

neg

n.a.

Faecal culture

I

40/ 51.9

37 /48.0

0 /0.0

39 /50.6

36 /46.8

2 /2.6

Faecal culture

II

41/ 53.2

36 /46.8

0 /0.0

38 /49.4

37 /48.0

2 /2.6

Faecal culture

III

41 /53.2

35 /45.5

1 /1.3

21 /27.3

54 /70.1

2 /2.6

qPCR A

II

35 /45.5

39 /50.6

3 /3.9

33 /42.9

35 /45.5

9 /11.7

qPCR B

III

37 /48.0

36 /46.8

4 /5.2

38 /49.4

32 /41.6

7 /9.1

  1. aLaboratory
  2. bMAP-positive
  3. cMAP-negative
  4. dNon-assessable faecal culture due to contamination or non-assessable qPCR results