Table 2 Phenotypical and genotypical properties of the investigated T. pyogenes
T. pyogenes 121,018,522 | T. pyogenes 121,008,157 | T. pyogenes 11-07-D 03394 | T. pyogenes DSM 20630T* | T. pyogenes DSM 20594* | |
|---|---|---|---|---|---|
Phenotypical properties | |||||
Hemolysis on sheep blood agar | + | + | + | + | + |
Hemolysis on rabbit blood agar | + | + | + | + | + |
CAMP-like reaction with: | |||||
Staphylococcus aureus β-hemolysin | + | + | + | + | + |
Streptococcus agalactiae | − | − | − | − | − |
Rhodococcus equi | + | + | + | + | + |
Reverse CAMP reaction | − | − | − | − | − |
Nitrate reduction1 | − | − | − | − | − |
Pyrazinamidase1 | − | − | − | − | − |
Pyrrolidonyl arylamidase1 | + | + | + | + | + |
Alkaline phosphatase1 | + | + | + | − | − |
β-Glucuronidase (β-GUR)1,2,3 | + | + | + | + | + |
α-Galactosidase (α-GAL)2 | − | − | − | − | − |
β-Galactosidase (β-GAL)1,3 | + | + | + | + | + |
α-Glucosidase (α-GLU)1,2,3 | + | + | + | + | + |
β-Glucosidase (β-GLU) | − | − | − | − | − |
N-acetyl- β-Glucosaminidase (β-NAG)1,3 | + | + | + | + | + |
Esculin (β-Glucosidase)1 | − | − | − | − | − |
Urease1 | − | − | − | − | − |
Gelatine1,4 | − | − | − | + | + |
Fermentation of: | |||||
D-Glucose1 | + | + | + | + | + |
D-Ribose1 | + | + | + | + | + |
D-Xylose1 | + | + | + | + | + |
D-Mannitol1 | − | − | − | − | − |
D-Maltose1 | + | + | + | + | + |
D-Lactose1 | + | + | + | + | + |
Glycogen1 | + | + | + | + | − |
α-Mannosidase2 | − | − | − | − | − |
Catalase | − | − | − | − | − |
Serolysis on Loeffler agar | + | + | + | + | + |
Caseinase | + | + | + | + | + |
Starch hydrolysis (amylase) | + | + | + | + | − |
Cross reaction with streptococcal serogroup G specific antiserum | + | + | + | + | + |
Genotypical properties | |||||
T. pyogenes 16S rRNA gene sequence | + | + | + | + | + |
T. pyogenes gene gap sequence | + | + | + | + | + |
T. pyogenes specific part of ISR | + | + | + | + | + |
T. pyogenes specific part of gene sodA | + | + | + | + | + |
Pyolysin encoding gene plo | + | + | + | + | + |
Collagen-binding protein encoding gene cbpA | − | − | − | + | − |
Neuraminidase H encoding gene nanH | + | + | + | + | + |
Neuraminidase P encoding gene nanP | − | − | − | + | + |
Fimbriae endoding gene fimA | + | + | + | − | + |
Fimbriae endoding gene fimC | + | + | + | + | + |
Fimbriae endoding gene fimE | + | + | + | + | + |
Tetracycline resistance encoding gene tet(W) | + | + | + | +** | −** |