The effect of dietary bacterial organic selenium on growth performance, antioxidant capacity, and Selenoproteins gene expression in broiler chickens

Table 2 Genes and primers used for relative quantification by real time PCR (qPCR) in the liver of chicken

Gene^a	Primer sequence (5′ − 3)^b	Fragment bp
GAPDH	Forward: 5′-AATGAGAGGTTCAGGTGCCC-3′	150
GAPDH	Reverse: 5′-ACCAGACAGCACTGTGTTGG-3′	150
GSH-Px1	Forward: 5′-GCGACTTCCTGCAGCTCAACGA-3′	99
GSH-Px1	Reverse: 5′-CGTTCTCCTGGTGCCCGAAT-3′	99
GSH-Px4	Forward: 5′-CGGTGAATTACACTCAGCTCGT-3′	123
GSH-Px4	Reverse: 5′-CTTTGATCTGCGCGTCGTCC-3′	123
DIO1	Forward: 5′-AAGCTGCACCTGACCTTCATT-3′	138
DIO1	Reverse: 5′-TTGTTTCTGAAGGCCCATCCA-3′	138
DIO2	Forward: 5′-CAGTGTAATCCACATAGCCA-3′	137
DIO2	Reverse: 5′-CTGAGCCAAAATTAACCACC-3′	137
SELW1	Forward: 5′-CTCCGCGTCACCGTGCTCT-3′	155
SELW1	Reverse: 5′-CTGCCCACCGTCACCTCGAAC-3′	155
TXNDR1	Forward: 5′-ACTGGATGACTATGACCGAA-3′	103
TXNDR1	Reverse: 5′-TATGCATTCTCATACGTGAC-3′	103

^a Abbreviation: GAPDH, glyceraldehyde-3-phosphate dehydrogenase; GSH-Px1,Glutathione peroxidase1; GSH-Px4, Glutathione peroxidase4; DIO1, iodothyronine deiodinase1; DIO2, iodothyronine deiodinase2; SELW1, selenoproteins w; TXNDR1, thioredoxin reductase
^bprimers used for qPCR designed based on pupplished sequences [54]

ISSN: 1746-6148