Fig. 3

Toxin neutralization assay (TNA)-titers. Displayed are toxin-neutralizing titers for individual animals as well as predicted means and standard deviations for group A (rBclA + rPA83), B (rBclA + rPA83 + FIS) and NegCTL (unvaccinated negative control) on all sampling points. Values below the detection limit (<50) were given an arbitrary value of . Individual values are given as symbols specific for each group (group A - triangle, group B - square and group NegCTL - circle). Means are given in the form of bar charts in the background and standard deviations as positive values above the bars. Results were analyzed for statistical differences with P ≤ 0.05 indicated by lower case letters for comparing time points and upper case letters for comparing groups above the respective bars. Mean values of different time points with identical lower case letters were not significant different from each other. Mean values of different groups with identical upper case letters were not significant different from each other. As there was no significant difference (P > 0.25) between group A and B for any sampling point concerning the toxin-neutralizing titers, letters were displayed across both groups. Mean values with identical letters were not significant different from each other. Means from group NegCTL and before immunization were always under the detection limit and therefore not included in the letter display. FIS: Formalin inactivated spores; rBcLA: Recombinant Bacillus collagen-like antigen; rPA: Recombinant protective antigen