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Table 2 TaqMan real time PCR result of detection of NDV AF2240 and IBS002 virus in the chicken bursa and IgM+ cells population isolated from chicken bursa of Fabricius

From: The effects of different velogenic NDV infections on the chicken bursa of Fabricius

 

Samples

C (t) mean

Viral copy number (log 10)

AF2240

IBS002

AF2240

IBS002

 

Control

N/D

N/D

N/D

N/D

Bursa of Fabricius

Day 1

N/D

N/D

N/D

N/D

Day 3

29.85 ± 0.28

26.24 ± 0.88

10.34*a

11.43*b

Day 4

28.33 ± 0.18

23.20 ± 0.53

10.80*a

12.34**b

IgM + cells

Control

N/D

N/D

N/D

N/D

Day 1

N/D

N/D

N/D

N/D

Day 3

35.10 ± 0.27

28.74 ± 0.03

8.76*a

10.68*b

Day 4

28.48 ± 0.16

26.82 ± 1.3

10.10**a

11.25**b

  1. N/D stands for not detectable. No amplification of NDV gene was detected throughout whole qPCR cycling program.
  2. All results represent the mean ± standard deviation
  3. Different asterisk signs show significant different between infection time points (P ≤ 0.05).
  4. Different alphabets show significant different between different strains of NDV (P ≤ 0.05).
  5. NDV AF2240, R2 = 0.984, Slope = 3.13