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Table 6 Comparison of observed quality parameters for the Gentian Canine CRP Immunoassay run on three different analyzers

From: Evaluation of a species-specific C-reactive protein assay for the dog on the ABX Pentra 400 clinical chemistry analyzer

Quality parameters Gentian CRP ABX Pentra 400 Abbott Architect c4000a Olympus AU600b
Limit of quantification (mg/l) 4.0c 6,8d 5.4e
Linearity (mg/l) 4–281 6.8–1201 ~5–100
Recovery (%) 90–105 116–123 105–118
No prozone effect (mg/l) ≤ 676 ≤ 1200 ≤ 400
Precision
CRP range (mg/l): < 270; > 25 < 270; > 25 ≤ 100, ≥ 50
Intra-assay CV (%) 0.7–1.9 0.5–1.7 1.0–1.3
Inter-assay CV (%)    
- Between run CV (%) 0.9–2.1 0.0–0.3 n.d.
- Between day CV (%) 1.1–4.6 1.1–1.9 4.1–4.7
No Interference up to
- Hemoglobin (g/l) 5 10 n.d.
- Bilirubin (mg/l) 800 n.d. n.d.
- Triglycerides (g/l) 10 10 n.d.
Method comparison
Reference method Randox Canine CRP assay Randox Canine CRP assay Olympus CRP
- rs 0.98 0.995 0.96
- intercept -1.18 7.3 n.d.
- slope 0.99 0.92 n.d.
- mean constant bias (%) 5.2 n.d. 41.0–70.5f
- TEobs (%) (~CRP 7–60 mg/l) 9.5–20.9 n.d. 50.4–82.6 f
(~CRP > 100 mg/l) 7.0–8.9 n.d. n.d
Sample type comparison small impact n.d. n.d.
  1. The assay was evaluated independently on the ABX Pentra 400 and compared to the data of the previous validation on the Abbott Architect c4000 and on the Olympus AU600.
  2. Abbreviations: n.d. not done, CV coefficient of variation, TE obs observed total allowable error
  3. a(Hillström et al. 2014) b(Muñoz-Prieto et al.2017)cbased on the desirable CV < 12.16%, dbased on desirable TE < 29.58%; both published in the addendum of the Total allowable error (TE) guidelines of the American College of Veterinary Clinical Pathology [32], ebased on CV < 20% (Escribano et al. 2012). f calculated based on the data of healthy and diseased dogs evaluated by Muñoz-Prieto et al.: healthy dogs: \( bias\%=\frac{median\ control- median\ test}{mean\ of\ the\ medians}=\frac{2.82\frac{mg}{l}-1.35\frac{mg}{l}}{\left(2.82-1.35\right):2} x100\%\approx 70.5\% \); TEobs = bias % + 2CV% = 70.5% + 2 × 6.05% = 82.6%
  4. dogs with inflammatory conditions:\( bias\%=\frac{73.7\frac{mg}{l}-48.6\frac{mg}{l}}{\left(73.7+48.6\right):2} x100\%\approx 41.0\% \);TEobs = 41.0% + 2 × 4.70% = 50.4%