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Table 4 Diagnostic sensitivity and specificity of the RespoCheck Mycoplasma triplex real-time PCR compared with the PCR/DGGE method

From: Mycoplasma detection by triplex real-time PCR in bronchoalveolar lavage fluid from bovine respiratory disease complex cases

M. dispar

PCR +

PCR -

Total

Diagnostic specificity and sensitivity

DGGE +

8

0

8

Sensitivity =1

DGGE -

29

7

36

Specificity =0.1944 (95% CI: 0.0819–0.3602)

Total

37

7

44

 

M. bovis

PCR +

PCR -

Total

Diagnostic specificity and sensitivity

DGGE +

20

1

21

Sensitivity =0.9524 (95% CI: 0.7618–0.9988)

DGGE -

6

17

23

Specificity =0.7391 (95% CI: 0.5159–0.8977)

Total

26

18

44

 

M. bovirhinis

PCR +

PCR -

Total

Diagnostic specificity and sensitivity

DGGE +

7

1

8

Sensitivity =0.8750 (95% CI: 0.4735–0.9968)

DGGE -

22

14

36

Specificity =0.3889 (95% CI: 0.2314–0.5654)

Total

29

15

44