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Fig. 2 | BMC Veterinary Research

Fig. 2

From: Mycoplasma detection by triplex real-time PCR in bronchoalveolar lavage fluid from bovine respiratory disease complex cases

Fig. 2

Analytical sensitivity of M. bovis, M. dispar and M. bovirhinis single and RespoCheck triplex real-time PCR in spiked BALF samples. Ten-fold serial dilutions of M. bovis, M. dispar and M. bovirhinis were made in BALF samples in a range from 3 × 106 down to 0.3 cfu/mL. The resulting samples were subjected to DNA isolation and testing in the M. bovis, M. dispar and M. bovirhinis single and RespoCheck triplex real-time PCR

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