Table 1 Summary of experimental design and biosecurity procedures followed prior to entry into the low, medium or high biosecurity rooms and the negative control room
From | To | Procedures | Trial 1 | Trial 2 |
|---|---|---|---|---|
INFECTED (INF) | Low Biosecurity (LB) | Direct movement from INF to LB through soiled corridor | 10 pigs | 4 pigs |
No change of clothes or footwear between INF and LB | 2 pigs/room | 4 pigs/room | ||
No washing of hands or face | 5 replicates | 1 replicate | ||
Medium Biosecurity (MB) | Movement from INF to MB through clean corridor only after procedures were followed | 10 pigs | 12 pigs | |
Wash hands and face | 2 pigs/room | 4 pigs/room | ||
Change clothes and footwear | 5 replicates | 3 replicates | ||
High Biosecurity (HB) | Movement from INF to HB through clean corridor only after procedures were followed | 10 pigs | NA | |
Shower | 2 pigs/room | NA | ||
Change clothes and footwear | 5 replicates | NA | ||
Negative control (NC) | No movement of people or fomities between INF. LB, MB or HB and negative control | 6 pigs | 3 pigs | |
Dedicated study personnel different from personnel attending the other groups | 6 pigs/room | 3 pigs/room | ||
Shower, clean clothes and footwear each time entering the room | 1 replicate | 1 replicate |