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Table 3 List of the primers used for the amplification of 3 overlapping fragments of 18S rDNA of piroplasms

From: First clinical case report of Cytauxzoon sp. infection in a domestic cat in France

Primer names

Primers sequences

Product size bp

PCR conditions

Reference

Nested PCR:

BTF1(external)

BTR1 (external)

GGCTCATTACAACAGTTATAG CCCAAAGACTTTGATTTCTCTC

930 bp

94 °C 3 min,

58 °C 1 min

72 °C 2 min

45 cycles:

94 °C 30s,

58 °C 20s

72 °C 30s

72 °C 7 min

[17]

BTF2 (internal)

BTR2 (internal)

CCGTGCTAATTGTAGGGCTAATAC GGACTACGACGGTATCTGATCG

836 bp

Same conditions for the secondary round with an annealing temperature of 62 °C

Paraseq1F_scanelis

18seq1R_scanelis

TGGCTCATTAMAACAGTTATAGTTTA AGACAAATCRCTCCACCAAC

1188

94 °C 3 min

45 cycles:

94 °C 20 s

56 °C 30 s

72 °C 45 s

72 °C 7 min

Unpublished

Piro-A

Piro-B

AAT ACC CAA TCC TGACAC AGG G TTA AAT ACG AAT GCC CCC AAC

408 bp

94 °C 1 min

39 cycles

94 °C 45 s,

62 °C 45 s,

72 °C 45 s.

72 °C 7 min

[18]