A novel real-time PCR assay for quantitative detection of Campylobacter fetus based on ribosomal sequences

Iraola, Gregorio; Pérez, Ruben; Betancor, Laura; Marandino, Ana; Morsella, Claudia; Méndez, Alejandra; Paolicchi, Fernando; Piccirillo, Alessandra; Tomás, Gonzalo; Velilla, Alejandra; Calleros, Lucía

doi:10.1186/s12917-016-0913-3

BMC Veterinary Research

Table 1 Isolates analyzed, discriminated by host, source, country and year of isolation

From: A novel real-time PCR assay for quantitative detection of Campylobacter fetus based on ribosomal sequences

Isolate	Host	Source	Country	Year	Phenotypic typing^a	Multiplex PCR A^b	Multiplex PCR B^c	Real-time PCR
A28	Bovine	U	Australia	1978	Cff	Cff	Cff	+
063	Bovine	Prepuce	Uruguay	1980	Cff	Cff	Cff	+
0835	Bovine	U	Uruguay	U	Cff	Cfv	Cff	+
F106	Bovine	U	Uruguay	U	Cff	Cff	Cff	+
71098	Bovine	Fetal abomasal content	Uruguay	1998	Cff	Cff	Cff	+
INTA 97/C1N3^d	Bovine	Vaginal mucus	Argentina	1997	Cff	Cff	Cff	+
INTA 04/554	Bovine	Fetal abomasal content	Argentina	2004	Cff	Cff	Cff	+
INTA 90/189	Bovine	Fetal lung	Argentina	1990	Cff	Cfv	Cfv	+
INTA 89/222	Bovine	Prepuce	Argentina	1989	Cff	No Cf/Cfv	No Cf/Cfv	+
INTA 01/165	Bovine	Vaginal mucus	Argentina	2001	Cff	Cff	Cff	+
INTA 12/218	Bovine	Fetal abomasal content	Argentina	2012	Cff	Cfv	Cfv	+
INTA 99/801	Bovine	Prepuce	Argentina	1999	Cff	Cff	Cff	+
INTA 01/064	Bovine	Vaginal mucus	Argentina	2001	Cff	Cff	Cff	+
INTA 04/875	Bovine	Vaginal mucus	Argentina	2004	Cff	Cff	Cff	+
INTA 08/328	Bovine	Fetal lung	Argentina	2008	Cff	Cff	Cff	+
INTA 05/622	Bovine	Fetal abomasal content	Argentina	2005	Cff	Cff	Cfv	+
INTA 11/262	Bovine	Fetal abomasal content	Argentina	2011	Cff	Cfv	Cfv	+
INTA 11/295	Bovine	Fetal abomasal content	Argentina	2011	Cff	Cfv	Cfv	+
INTA 11/685A	Bovine	Vaginal mucus	Argentina	2011	Cff	Cfv	Cff	+
INTA 11/685B	Bovine	Fetal abomasal content	Argentina	2011	Cff	Cfv	Cff	+
INTA 11/677	Bovine	Fetal abomasal content	Argentina	2011	Cff	Cff	Cff	+
INTA 11/501	Bovine	Vaginal mucus	Argentina	2011	Cff	Cff	Cff	+
INTA 11/408	Bovine	Fetal abomasal content	Argentina	2011	Cff	Cff	Cff	+
INTA 11/356	Bovine	Fetal abomasal content	Argentina	2011	Cff	Cff	Cfv	+
INTA 11/360	Bovine	Fetal lung	Argentina	2011	Cff	Cfv	Cfv	+
NCTC10354^T	Bovine	U	England	1962	Cfv	Cff	Cfv	+
D78	Bovine	U	Australia	1978	Cfv	Cfv	Cfv	+
660	Bovine	Fetal abomasal content	Uruguay	2010	Cfv	Cfv	Cfv	+
3726	Bovine	Fetal abomasal content	Uruguay	2010	Cfv	Cfv	Cfv	+
2733	Bovine	Fetal abomasal content	Uruguay	2006	Cfv	Cfv	Cfv	+
2740	Bovine	Fetal abomasal content	Uruguay	2006	Cfv	Cfv	Cfv	+
MCR03	Bovine	Prepuce	Uruguay	2009	Cfv	Cfv	Cfv	+
3837	Bovine	Fetal abomasal content	Uruguay	2010	Cfv	Cfv	Cfv	+
1198	Bovine	U	Uruguay	U	Cfv	Cff	Cfv	+
3598	Bovine	U	Uruguay	U	Cfv	Cff	Cfv	+
2432	Bovine	U	Uruguay	2010	Cfv	Cfv	Cfv	+
2370P	Bovine	Fetal abomasal content	Uruguay	2011	Cfv	Cfv	Cfv	+
2374C	Bovine	Fetal abomasal content	Uruguay	2011	Cfv	Cfv	Cfv	+
27460P	Bovine	Fetal abomasal content	Uruguay	2011	Cfv	Cfv	Cfv	+
INTA 97/608^d	Bovine	Placenta	Argentina	1997	Cfv	Cfv	Cfv	+
INTA 83/371	Bovine	Vaginal mucus	Argentina	1983	Cfv	Cfv	Cfv	+
INTA 90/264	Bovine	Fetal abomasal content	Argentina	1990	Cfv	Cff	Cfv	+
INTA 05/355	Bovine	Fetal abomasal content	Argentina	2005	Cfv	Cfv	Cfv	+
INTA 95/258	Bovine	Vaginal mucus	Argentina	1995	Cfv	Cff	Cfv	+
INTA 08/382	Bovine	Fetal abomasal content	Argentina	2008	Cfv	Cff	Cfv	+
021	Bovine	U	Australia	1978	Cfvi	Cfv	Cfv	+
INTA 98/BL472	Bovine	Fetal abomasal content	Argentina	1998	Cfvi	Cfv	Cfv	+
INTA 99/541	Bovine	Prepuce	Argentina	1999	Cfvi	Cff	Cfv	+
INTA 97/384	Bovine	Fetal abomasal content	Argentina	1997	Cfvi	Cff	Cfv	+
INTA 98/472	Bovine	Fetal abomasal content	Argentina	1998	Cfvi	Cfv	Cfv	+
INTA 00/305	Bovine	Fetal abomasal content	Argentina	2000	Cfvi	Cff	Cfv	+
INTA 02/146	Bovine	Vaginal mucus	Argentina	2002	Cfvi	Cfv	Cfv	+
INTA 03/596	Bovine	Fetal abomasal content	Argentina	2003	Cfvi	Cff	Cff	+
INTA 07/379	Bovine	Fetal abomasal content	Argentina	2007	Cfvi	Cff	Cfv	+
INTA 06/341	Bovine	Fetal lung	Argentina	2006	Cfvi	Cfv	Cfv	+
H1-UY	Human	Blood	Uruguay	2013	Cf	Cff	Cff	+
HC	Human	Blood	Uruguay	2014	Cf	Cff	Cff	+
70 L	Human	Cerebrospinal fluid	Uruguay	2014	Cf	Cff	Cff	+
70H	Human	Blood	Uruguay	2014	Cf	Cff	Cff	+
RA8/Italy/2011	Turtle	Cloaca	Italy	2011	Cft	No Cf	No Cf	+
RC7	Turtle	Cloaca	Italy	2011	C. geochelonis	No Cf	No Cf	-
RC11	Turtle	Cloaca	Italy	2011	C. geochelonis	No Cf	No Cf	-
RC20	Turtle	Cloaca	Italy	2011	C. geochelonis	No Cf	No Cf	-
INTA 08/209	Bovine	Prepuce	Argentina	2008	C. sputorum	No Cf	No Cf	-
CcHB41	Human	Feces	Uruguay	2010	C. coli	No Cf	No Cf	-
CjHB32	Human	Feces	Uruguay	2010	C. jejuni	No Cf	No Cf	-
CjCP3	Chicken	Cecal content	Uruguay	2010	C. jejuni	No Cf	No Cf	-
CcCP60	Chicken	Cecal content	Uruguay	2009	C. coli	No Cf	No Cf	-
INTA 99/243	U	Vaginal mucus	Argentina	1999	C. hyointestinalis	No Cf	No Cf	-
NCTC 11562	Pork	U	England	1983	C. hyointestinalis	No Cf	No Cf	-

Cft Campylobacter fetus subsp. testudinum, Cff Campylobacter fetus subsp. fetus, Cfv Campylobacter fetus subsp. venerealis, Cfvi Campylobacter fetus subsp. venerealis biovar intermedius, Cf Campylobacter fetus, U unknown, ND not determined
^ain C. fetus, glycine tolerance and H2S production, see text for details
^bAs described in Hum et al. [12]
^cAs described in Iraola et al. [41]
^dThese strains were assayed both starting from a resuspended culture and directly from bovine samples of placenta or vaginal mucus, without a previous isolation step

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