Table 2 Primers and probe set used in RT-qPCR assays
5′ Fluorophore | 3′ Quencher | Sequences (5′–3′) | Amplicon size | Annealing (°C) | ||
|---|---|---|---|---|---|---|
DMV-N-FP | – | – | TGCCAGTACTCCAGGGAACATCCTTC | 173 | 60 | [26] |
DMV-N-RP | – | – | TTGGGTCGTCAGTGTTGTCGGACCGTT | 173 | 60 | |
DMV-N-probe | Cy3 | BHQ1 | A + CA + CCAAA + AGGGA + CA | – | 60 |