Fig. 2
From: Classical swine fever virus replicated poorly in cells from MxA transgenic pigs
a-b. PCR data for the neomycin resistance gene (Neo), and RT-PCR data for MxA were performed to confirm the presence of the transgene; the No. 34 clone cells were chosen for the positive control, and normal porcine tails gDNA and blood RNA were chosen as negative control. c. Southern blotting was performed to detect the vector fragment in the genome; pGKneotAloxp2MxA was chosen as the positive control. d: Western blotting showed the expression of MxA in all three piglets; a cell strain stably expressing MxA was chosen for the positive control, and tissue from non-transgenic piglets was chosen as a negative control