Fig. 4

Immunofluorescent findings for the detection of GM1 ganglioside in animals affected and unaffected with gangliosidoses. The immunofluorescent technique for the detection of GM1 ganglioside was performed on paraffin-embedded sections of the cerebral cortex from the following animals: a dog (a) and a cat (b) affected with GM1 gangliosidosis; a dog (c) and a cat (d) affected with GM2 gangliosidosis; an unaffected control dog (e) and cat (f). For the detection of GM1 ganglioside, biotinylated cholera toxin B subunit and Alexa Fluor 488-conjugated streptavidin were used. Subsequently, these sections were incubated with 4′,6-diamidino-2-phenylindole dihydrochloride for nuclear staining. Bar = 30 μm